Exploiting unassigned codons in Micrococcus luteus for tRNA-based amino acid mutagenesis
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چکیده
منابع مشابه
Exploiting unassigned codons in Micrococcus luteus for tRNA-based amino acid mutagenesis.
An alternative to suppression of stop codons for the biosynthetic insertion of non-natural amino acids has been developed. Micrococcus luteus , a Gram-positive bacterium, is incapable of translating at least two codons. One of these unused codons was inserted in a gene to act as a nonsense site. An aminoacylated tRNA was synthesized which was complementary to this codon. The gene containing the...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 1362-4962
DOI: 10.1093/nar/25.22.4685